[HTML][HTML] Bacterial lipopolysaccharide induces expression of the stress response genes hop and H411
CD14-transfected Chinese hamster ovary K1 fibroblasts (CHO/CD14) respond to
lipopolysaccharide (LPS) by metabolizing arachidonic acid and with translocation of NF-κB
to the nucleus. Although previous experiments failed to identify the production of tumor
necrosis factor-α and interleukin (IL)-1β by CHO/CD14 cells, LPS did induce the expression
of IL-6 mRNA and the subsequent release of the IL-6 protein. To identify additional LPS-
inducible genes, a cDNA library derived from LPS-stimulated CHO/CD14 cells was …
lipopolysaccharide (LPS) by metabolizing arachidonic acid and with translocation of NF-κB
to the nucleus. Although previous experiments failed to identify the production of tumor
necrosis factor-α and interleukin (IL)-1β by CHO/CD14 cells, LPS did induce the expression
of IL-6 mRNA and the subsequent release of the IL-6 protein. To identify additional LPS-
inducible genes, a cDNA library derived from LPS-stimulated CHO/CD14 cells was …