[5] Preparation and properties of succinate: Ubiquinone oxidoreductase (complex II)
Y Hatefi, DL Stiggall - Methods in enzymology, 1978 - Elsevier
Publisher Summary This chapter describes the properties, preparation, and purification
procedure of ubiquinone oxidoreductase (complex II) enzyme. The preparation is carried out
essentially according to published procedures, but with modifications that result in better
reproducibility. The pH values of buffers, deoxycholate, and cholate solutions are adjusted at
20°–25°. Complex II is the segment of the respiratory chain that catalyzes the transfer of
reducing equivalents from succinate to ubiquinone. Succinate dehydrogenase constitutes …
procedure of ubiquinone oxidoreductase (complex II) enzyme. The preparation is carried out
essentially according to published procedures, but with modifications that result in better
reproducibility. The pH values of buffers, deoxycholate, and cholate solutions are adjusted at
20°–25°. Complex II is the segment of the respiratory chain that catalyzes the transfer of
reducing equivalents from succinate to ubiquinone. Succinate dehydrogenase constitutes …