[HTML][HTML] A novel 160-kDa phosphotyrosine protein in insulin-treated embryonic kidney cells is a new member of the insulin receptor substrate family
We have previously identified a 160-kDa protein in human embryonic kidney (HEK) 293
cells that undergoes rapid tyrosine phosphorylation in response to insulin (PY160)(Kuhné,
MR, Zhao, Z., and Lienhard, GE (1995) Biochem. Biophys. Res. Commun. 211, 190–197).
The phosphotyrosine form of PY160 was purified from insulin-treated HEK 293 cells by anti-
phosphotyrosine immunoaffinity chromatography, the sequences of peptides determined,
and its cDNA cloned. The PY160 cDNA encodes a 1257-amino acid protein that contains, in …
cells that undergoes rapid tyrosine phosphorylation in response to insulin (PY160)(Kuhné,
MR, Zhao, Z., and Lienhard, GE (1995) Biochem. Biophys. Res. Commun. 211, 190–197).
The phosphotyrosine form of PY160 was purified from insulin-treated HEK 293 cells by anti-
phosphotyrosine immunoaffinity chromatography, the sequences of peptides determined,
and its cDNA cloned. The PY160 cDNA encodes a 1257-amino acid protein that contains, in …