Metabolism of apoE-free high density lipoproteins in rat hepatoma cells: evidence for a retroendocytic pathway.
JG DeLamatre, TG Sarphie, RC Archibold… - Journal of Lipid …, 1990 - ASBMB
The cellular metabolism of apoE-free HDL (HDL) was studied in rat hepatoma cells
(FU5AH). Cells incubated with HDL showed a dose-dependent decreased incorporation of
[14C] acetate into cell sterol, indicating a net cholesterol delivery to the cells. HDL was
localized both at the cell surface and inside the cell. This conclusion was drawn from both
the association of 125I-labeled HDL with the cells under different experimental conditions
and morphological evidence based on the association of colloidal gold-labeled HDL with …
(FU5AH). Cells incubated with HDL showed a dose-dependent decreased incorporation of
[14C] acetate into cell sterol, indicating a net cholesterol delivery to the cells. HDL was
localized both at the cell surface and inside the cell. This conclusion was drawn from both
the association of 125I-labeled HDL with the cells under different experimental conditions
and morphological evidence based on the association of colloidal gold-labeled HDL with …
