[HTML][HTML] β-Glucocerebrosidase activity in mammalian stratum corneum
Y Takagi, E Kriehuber, G Imokawa, PM Elias… - Journal of lipid …, 1999 - ASBMB
Although previous studies have demonstrated a crucial role for the enzyme β-
glucocerebrosidase (GlcCer'ase) in the final steps of membrane structural maturation in
mammalian stratum cornuem (SC) and epidermal homeostasis, the precise in vivo
localization of GlcCer'ase activity and protein is not known. Here, we developed a
fluorogenic in situ assay on histologic sections (zymography) to elucidate the in vivo
distribution of GlcCer'ase activity, and further characterized and localized the SC GlcCer'ase …
glucocerebrosidase (GlcCer'ase) in the final steps of membrane structural maturation in
mammalian stratum cornuem (SC) and epidermal homeostasis, the precise in vivo
localization of GlcCer'ase activity and protein is not known. Here, we developed a
fluorogenic in situ assay on histologic sections (zymography) to elucidate the in vivo
distribution of GlcCer'ase activity, and further characterized and localized the SC GlcCer'ase …