Calcineurin mediates inhibition by FK506 and cyclosporin of recovery from α-factor arrest in yeast

F Poor, SA Parent, N Morin, AM Dahl, N Ramadan… - Nature, 1992 - nature.com
F Poor, SA Parent, N Morin, AM Dahl, N Ramadan, G Chrebet, KA Bostian, JB Nielsen
Nature, 1992nature.com
THE structurally unrelated immunosuppressants FK506 and cyclosporin A (CsA) act
similarly, inhibiting a Ca2+-dependent signal required for interleukin-2 transcription and T-
cell activation1. Each drug binds to its cytosolic receptor, FKBP-12 and cyclophilin,
respectively, and the drug-receptor complexes inhibit the Ca2+/calmodulin-dependent
protein phosphatase, calcineurin2–4. In yeast, calcineurin has been implicated in recovery
from α-mating factor arrest5, 6. Here we show that FK506 bound to yeast FKBP-12 appears …
Abstract
THE structurally unrelated immunosuppressants FK506 and cyclosporin A (CsA) act similarly, inhibiting a Ca2+-dependent signal required for interleukin-2 transcription and T-cell activation1. Each drug binds to its cytosolic receptor, FKBP-12 and cyclophilin, respectively, and the drug-receptor complexes inhibit the Ca2+/calmodulin-dependent protein phosphatase, calcineurin2–4. In yeast, calcineurin has been implicated in recovery from α-mating factor arrest5,6. Here we show that FK506 bound to yeast FKBP-12 appears to form a complex with yeast calcineurin. Moreover, recovery from mating factor arrest is highly sensitive to FK506 or CsA, and this sensitivity requires the presence of FKBP-12 or cyclophilin, respectively. These results define a key physiological target of an FK506- and CsA-sensitive signal pathway in yeast, suggest a high degree of mechanistic conservation with mammalian cells, and indicate that further examination of the yeast system should provide insight into the same process in T cells.
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