[HTML][HTML] The Kinetics, Substrate, and Inhibitor Specificity of the Monocarboxylate (Lactate) Transporter of Rat Liver Cells Determined Using the Fluorescent …
VN Jackson, AP Halestrap - Journal of Biological Chemistry, 1996 - ASBMB
The kinetics of transport of L-lactate, pyruvate, ketone bodies, and other monocarboxylates
into isolated hepatocytes from starved rats were measured at 25° C using the intracellular
pH-sensitive dye, 2′, 7′-bis (carboxyethyl)-5 (6)-carboxyfluorescein, to detect the
associated proton influx. Transport kinetics were similar, but not identical, to those
determined using the same technique for the monocarboxylate transporter (MCT) of Ehrlich
Lettré tumor cells (MCT1)(Carpenter, L., and Halestrap, AP (1994) Biochem. J. 304, 751 …
into isolated hepatocytes from starved rats were measured at 25° C using the intracellular
pH-sensitive dye, 2′, 7′-bis (carboxyethyl)-5 (6)-carboxyfluorescein, to detect the
associated proton influx. Transport kinetics were similar, but not identical, to those
determined using the same technique for the monocarboxylate transporter (MCT) of Ehrlich
Lettré tumor cells (MCT1)(Carpenter, L., and Halestrap, AP (1994) Biochem. J. 304, 751 …