[HTML][HTML] Mechanical strain increases expression of the brain natriuretic peptide gene in rat cardiac myocytes
F Liang, J Wu, M Garami, DG Gardner - Journal of Biological Chemistry, 1997 - ASBMB
Using a device that applies cyclical strain (1 Hz) to ventricular cardiocytes cultured on
collagen-coated silicone elastomer surfaces, we have demonstrated strain-dependent
increases in brain natriuretic peptide (BNP) secretion, BNP mRNA levels, and expression of
a transiently transfected− 1595 human BNP-luciferase reporter. When actinomycin D (10
μm) was introduced concomitantly with the strain stimulus, the strain-induced increase in
BNP mRNA was eliminated, and the decay of transcripts was identical in the control and …
collagen-coated silicone elastomer surfaces, we have demonstrated strain-dependent
increases in brain natriuretic peptide (BNP) secretion, BNP mRNA levels, and expression of
a transiently transfected− 1595 human BNP-luciferase reporter. When actinomycin D (10
μm) was introduced concomitantly with the strain stimulus, the strain-induced increase in
BNP mRNA was eliminated, and the decay of transcripts was identical in the control and …