Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.
PM Handley, M Mueckler, NR Siegel… - Proceedings of the …, 1991 - National Acad Sciences
PM Handley, M Mueckler, NR Siegel, A Ciechanover, AL Schwartz
Proceedings of the National Academy of Sciences, 1991•National Acad SciencesThe ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have
cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da.
Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein
E2, suggesting a potential role for this region in enzymatic function of this protein. In
addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of
several proteins involved in thioester formation. Tissue distribution reveals a single 3.5 …
cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da.
Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein
E2, suggesting a potential role for this region in enzymatic function of this protein. In
addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of
several proteins involved in thioester formation. Tissue distribution reveals a single 3.5 …
The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.
National Acad Sciences