Role of Two Acetylcholine Receptor Subunit Domains in Homomer Formation and Intersubunit Recognition, as Revealed by. alpha. 3 and. alpha. 7 Subunit Chimeras
M Garcia-Guzman, F Sala, S Sala, A Campos-Caro… - Biochemistry, 1994 - ACS Publications
M Garcia-Guzman, F Sala, S Sala, A Campos-Caro, M Criado
Biochemistry, 1994•ACS PublicationsMATERIALS AND METHODS Construction of Chimeras. When possible, chimeric assembly
was performed using restriction sites common to both subunits {Sacl and Narl, see Figure 1,
al subunit). Other chimerasand point mutations were made, essentially as described
previously (Herlitze& Koenen, 1990), by performing two successive PCR amplifications. In
the first PCR (20 cycles: 94 C for 1 min; 48 C for 1 min; 72 C
was performed using restriction sites common to both subunits {Sacl and Narl, see Figure 1,
al subunit). Other chimerasand point mutations were made, essentially as described
previously (Herlitze& Koenen, 1990), by performing two successive PCR amplifications. In
the first PCR (20 cycles: 94 C for 1 min; 48 C for 1 min; 72 C
MATERIALS AND METHODS
Construction of Chimeras. When possible, chimeric assembly was performed using restriction sites common to both subunits {Sacl and Narl, see Figure 1, al subunit). Other chimerasand point mutations were made, essentially as described previously (Herlitze& Koenen, 1990), by performing two successive PCR amplifications. In the first PCR (20 cycles: 94 C for 1 min; 48 C for 1 min; 72 C
ACS Publications