Epithelial-mesenchymal transformation of embryonic cardiac endothelial cells is inhibited by a modified antisense oligodeoxynucleotide to transforming growth factor …
JD Potts, JM Dagle, JA Walder… - Proceedings of the …, 1991 - National Acad Sciences
Proceedings of the National Academy of Sciences, 1991•National Acad Sciences
During early cardiac development, the progenitor cells of the heart valves and membranous
septa undergo an epithelial-mesenchymal transformation. Previous studies have shown that
this transformation depends on the activity of a transforming growth factor beta (TGF beta)
molecule produced by the heart. In the present study, we have used modified antisense
oligodeoxynucleotides generated to nonconserved regions of TGF beta 1,-2,-3, and-4 to
examine the possible roles of these members in this transformation. A phosphoramidate …
septa undergo an epithelial-mesenchymal transformation. Previous studies have shown that
this transformation depends on the activity of a transforming growth factor beta (TGF beta)
molecule produced by the heart. In the present study, we have used modified antisense
oligodeoxynucleotides generated to nonconserved regions of TGF beta 1,-2,-3, and-4 to
examine the possible roles of these members in this transformation. A phosphoramidate …
During early cardiac development, the progenitor cells of the heart valves and membranous septa undergo an epithelial-mesenchymal transformation. Previous studies have shown that this transformation depends on the activity of a transforming growth factor beta (TGF beta) molecule produced by the heart. In the present study, we have used modified antisense oligodeoxynucleotides generated to nonconserved regions of TGF beta 1, -2, -3, and -4 to examine the possible roles of these members in this transformation. A phosphoramidate-modified oligonucleotide complementary to TGF beta 3 mRNA was capable of inhibiting normal epithelial-mesenchymal transformation by 80%. Unmodified oligonucleotides to TGF beta 3, modified oligonucleotides to TGF beta 1, -2, and -4, and two modified control oligonucleotides were unable to inhibit the transformation. These data demonstrate that a specific member of the TGF beta family, TGF beta 3, is essential for the epithelial-mesenchymal cell transformation.
National Acad Sciences