Immunochemical studies on acetylcholine receptor from Torpedo californica
A Aharonov, R Tarrab-Hazdai, I Silman, S Fuchs - Immunochemistry, 1977 - Elsevier
Acetylcholine receptor (AChR) was purified from electric organ tissue of Torpedo california
by solubilization of membrane fragments with 1% Triton X-100 followed by affinity
chromatography on a Naja naja siamensis neurotoxin-Sepharose resi. The molecular and
pharmacological properties of the purified recerptor were determined, and it was shown to
have a specific activity of 8–12 nmoles toxin bound per mg protein, corresponding to 80,000–
125,000 daltons per toxin-binding site. Experimental autoimmune myasthenia gravis was …
by solubilization of membrane fragments with 1% Triton X-100 followed by affinity
chromatography on a Naja naja siamensis neurotoxin-Sepharose resi. The molecular and
pharmacological properties of the purified recerptor were determined, and it was shown to
have a specific activity of 8–12 nmoles toxin bound per mg protein, corresponding to 80,000–
125,000 daltons per toxin-binding site. Experimental autoimmune myasthenia gravis was …