The predicted protein domains coded by exons 9–12 and 19–23 of the 27 exon cystic fibrosis transmembrane conductance regulator (CFTR) gene contain two putative nucleotide‐binding fold regions. Analysis of CFTR mRNA transcripts in freshly isolated bronchial epithelium from 12 normal adult individuals demonstrated that all had some CFTR mRNA transcripts with exon 9 completely deleted (exon 9‐ mRNA transcripts). In most (9 of 12), the exon 9‐ transcripts represented less than or equal to 25% of the total CFTR transcripts. However, in three individuals, the exon 9‐ transcripts were more abundant, comprising 39, 62 and 66% of all CFTR transcripts. Re‐evaluation of the same individuals 2–4 months later showed the same proportions of exon 9‐ transcripts. Of the 24 CFTR alleles in the 12 individuals, the sequences of the exon‐intron junctions relevant to exon 9 deletion (exon 8‐intron 8, intron 8‐exon 9, exon 9‐intron 9, and intron 9‐exon 10) were identical except for the intron 8‐exon 9 region sequences. Several individuals had varying lengths of a TG repeat in the region between splice branch and splice acceptor consensus sites. Interestingly, one allele in each of the two individuals with 62 and 66% exon 9‐ transcripts had a TT deletion in the splice acceptor site for exon 9. These observations suggest either the unlikely possibility that sequences in exon 9 are not critical for the functioning of the CFTR or that only a minority of the CFTR mRNA transcripts need to contain exon 9 sequences to produce sufficient amounts of a normal CFTR to maintain a normal clinical phenotype.