Purification and characterization of rat liver microsomal thiol methyltransferase
RT Borchardt, CF Cheng - Biochimica et Biophysica Acta (BBA) …, 1978 - Elsevier
Thiol methyltransferase (S-Adenosyl-l-methionine: thiol S-methyltransferase, EC 2.1. 1.9), an
enzyme which catalyzes the transfer of a methyl group from S-adenosylmethionine to the
sulfhydryl group of a methyl acceptor, has been solubilized and partially purified from rat
liver microsomes. The enzyme methylates xenobiotic sulfhydryl-containing compounds (eg β-
mercaptoethanol), but not physiological sulfhydryl-containing compounds (eg homocysteine,
cysteine, etc.). This thiol methyltransferase was inhibited by N-ethylmaleimide and p …
enzyme which catalyzes the transfer of a methyl group from S-adenosylmethionine to the
sulfhydryl group of a methyl acceptor, has been solubilized and partially purified from rat
liver microsomes. The enzyme methylates xenobiotic sulfhydryl-containing compounds (eg β-
mercaptoethanol), but not physiological sulfhydryl-containing compounds (eg homocysteine,
cysteine, etc.). This thiol methyltransferase was inhibited by N-ethylmaleimide and p …
