Detection of rhinovirus, respiratory syncytial virus, and coronavirus infections in acute otitis media by reverse transcriptase polymerase chain reaction
A Pitkäranta, A Virolainen, J Jero, E Arruda… - …, 1998 - publications.aap.org
Objective. To determine the frequencies of human rhinovirus (HRV), respiratory syncytial
virus (RSV), and coronavirus (HCV) infection in children with acute otitis media (AOM).
Methods. Middle ear fluids (MEF) collected by tympanocentesis and nasopharyngeal
aspirates (NPA) at the time of the AOM diagnosis were examined by reverse transcriptase
polymerase chain reaction for HRV, RSV, and HCV RNA. Patients. Ninety-two children aged
3 months to 7 years during a 1-year period. Results. Virus RNA was detected in a total of 69 …
virus (RSV), and coronavirus (HCV) infection in children with acute otitis media (AOM).
Methods. Middle ear fluids (MEF) collected by tympanocentesis and nasopharyngeal
aspirates (NPA) at the time of the AOM diagnosis were examined by reverse transcriptase
polymerase chain reaction for HRV, RSV, and HCV RNA. Patients. Ninety-two children aged
3 months to 7 years during a 1-year period. Results. Virus RNA was detected in a total of 69 …
