[HTML][HTML] Saturation of the endocytic pathway for the transferrin receptor does not affect the endocytosis of the epidermal growth factor receptor
RA Warren, FA Green, CA Enns - Journal of Biological Chemistry, 1997 - ASBMB
Cell-surface receptors that undergo clathrin-mediated endocytosis contain short amino acid
sequences in their cytoplasmic domain that serve as internalization signals. Interactions
between these sequences and components of the endocytic machinery should become
limiting upon overexpression of the constitutively recycling transferrin receptor (TfR). A
tetracycline-responsive system was used to induce overexpression of the TfR up to 20-fold
in HeLa cells. Internalization assays indicate the rate of 125 I-transferrin uptake per surface …
sequences in their cytoplasmic domain that serve as internalization signals. Interactions
between these sequences and components of the endocytic machinery should become
limiting upon overexpression of the constitutively recycling transferrin receptor (TfR). A
tetracycline-responsive system was used to induce overexpression of the TfR up to 20-fold
in HeLa cells. Internalization assays indicate the rate of 125 I-transferrin uptake per surface …