[HTML][HTML] Insulin-like growth factor 1 inhibits apoptosis using the phosphatidylinositol 3′-kinase and mitogen-activated protein kinase pathways
M Párrizas, AR Saltiel, D LeRoith - Journal of Biological Chemistry, 1997 - ASBMB
The role of insulin-like growth factor 1 (IGF-1) in preventing apoptosis was examined in
differentiated PC12 cells. Induction of differentiation was achieved using nerve growth factor,
and apoptosis was provoked by serum withdrawal. After 4-6 h of serum deprivation,
apoptosis was initiated, concomitant with a 30% decrease in cell number and a 75%
decrease in MTT activity. IGF-1 was capable of preventing apoptosis at concentrations as
low as 10− 9 M and as early as 4 h. The phosphatidylinositol 3′(PI3′)-kinase inhibitors …
differentiated PC12 cells. Induction of differentiation was achieved using nerve growth factor,
and apoptosis was provoked by serum withdrawal. After 4-6 h of serum deprivation,
apoptosis was initiated, concomitant with a 30% decrease in cell number and a 75%
decrease in MTT activity. IGF-1 was capable of preventing apoptosis at concentrations as
low as 10− 9 M and as early as 4 h. The phosphatidylinositol 3′(PI3′)-kinase inhibitors …