Specificity of a monoclonal antibody for the NH2-terminal region of fibrin
B Kudryk, A Rohoza, M Ahadi, J Chin, ME Wiebe - Molecular immunology, 1984 - Elsevier
B Kudryk, A Rohoza, M Ahadi, J Chin, ME Wiebe
Molecular immunology, 1984•ElsevierA monoclonal antibody (MAb/T2G1s) was prepared by fusion using spleen cells from mice
immunized with the NH 2-terminal CNBr fragment of human fibrin II, the so-called (T) N-DSK
[(Aα 17–51, Bβ 15–118, γ 1–78) 2]. In competition experiments, this antibody reacted with (T)
N-DSK as well as peptide Bβ 15–42 which can be obtained from (T) N-DSK by digestion
with plasmin. Little or no reaction was observed with intact fibrinogen, the NH 2-terminal
CNBr fragments from fibrinogen (N-DSK) or fibrin I [(B) N-DSK], respectively, as well as …
immunized with the NH 2-terminal CNBr fragment of human fibrin II, the so-called (T) N-DSK
[(Aα 17–51, Bβ 15–118, γ 1–78) 2]. In competition experiments, this antibody reacted with (T)
N-DSK as well as peptide Bβ 15–42 which can be obtained from (T) N-DSK by digestion
with plasmin. Little or no reaction was observed with intact fibrinogen, the NH 2-terminal
CNBr fragments from fibrinogen (N-DSK) or fibrin I [(B) N-DSK], respectively, as well as …
Abstract
A monoclonal antibody (MAb/T2G1s) was prepared by fusion using spleen cells from mice immunized with the NH2-terminal CNBr fragment of human fibrin II, the so-called (T)N-DSK [(Aα 17–51, Bβ 15–118, γ 1–78)2]. In competition experiments, this antibody reacted with (T)N-DSK as well as peptide Bβ 15–42 which can be obtained from (T)N-DSK by digestion with plasmin. Little or no reaction was observed with intact fibrinogen, the NH2-terminal CNBr fragments from fibrinogen (N-DSK) or fibrin I [(B)N-DSK], respectively, as well as peptide Bβ 1–42. These results suggest that MAb/T2G1s is directed to an epitope on the Bβ chain in fibrin II but not in fibrinogen or fibrin I. As such, MAb/T2G1s differs completely from another antibody (MAb/1-8C6)—also specific for the NH2-terminal region of the Bβ chain—which was recently described [Kudryk et al. (1983) Molec. Immun.20, 1191–1200].
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