[HTML][HTML] Uni-axial cyclic stretch induces c-src activation and translocation in human endothelial cells via SA channel activation

K Naruse, X Sai, N Yokoyama, M Sokabe - FEBS letters, 1998 - Elsevier
K Naruse, X Sai, N Yokoyama, M Sokabe
FEBS letters, 1998Elsevier
The kinase activity of c-src increased and peaked at 15 min after an application of uni-axial
cyclic stretch in HUVECs followed by a translocation of c-src to Triton-insoluble fraction.
Suppression of c-src by an antisense S-oligodeoxynucleotide inhibited the stretch-induced
tyrosine phosphorylation and morphological changes. The stretch-induced increase in c-src
activity was inhibited by FK506, a specific inhibitor for calcineurin, by Gd3+, a blocker for
stretch activated channels, and by the extracellular Ca2+ depletion suggesting the …
The kinase activity of c-src increased and peaked at 15 min after an application of uni-axial cyclic stretch in HUVECs followed by a translocation of c-src to Triton-insoluble fraction. Suppression of c-src by an antisense S-oligodeoxynucleotide inhibited the stretch-induced tyrosine phosphorylation and morphological changes. The stretch-induced increase in c-src activity was inhibited by FK506, a specific inhibitor for calcineurin, by Gd3+, a blocker for stretch activated channels, and by the extracellular Ca2+ depletion suggesting the involvement of SA channels. These results strongly suggest c-src plays an important role in the downstream of SA channel activation followed by the morphological changes.
Elsevier