[HTML][HTML] Dual reactivity of several monoclonal anti-nucleosome autoantibodies for double-stranded DNA and a short segment of histone H3

C Stemmer, P Richalet-Sécordel… - Journal of Biological …, 1996 - ASBMB
We have shown previously that four IgG monoclonal autoantibodies (mAbs) reacted in
ELISA with both double-stranded (ds) DNA and peptide 83-100 of histone H3. The peptide
83-100 contains a cysteine residue at position 96 and readily dimerizes at pH 7-8. We
describe here that only the 83-100 dimers, and not the 83-100 monomers, are recognized by
the four antibodies and inhibit in ELISA the binding of mAbs to dsDNA. The equilibrium
affinity constants (K a) and kinetic rate constants of two of these mAbs were measured in a …