Use of recombinant adenovirus for metabolic engineering of mammalian cells

TC Becker, RJ Noel, WS Coats, AM Gomez-Foix… - Methods in cell …, 1994 - Elsevier
Publisher Summary This chapter describes the utility of Adenovirus for transfer of genes
involved in metabolic regulation into mammalian cells, with particular emphasis to primary
cell types with low replicative activity, such as hepatocytes and cells of the islets of
Langerhans. The chapter provides methods and procedures for constructing and
propagating new recombinant virions. Recombinant adenoviruses have been useful for
delivering genes to whole animals. Viruses have been administered as single injections via …