Structural analysis of γδ TCR using a novel set of TCR γ and δ chain-specific monoclonal antibodies generated against soluble γδ TCR: evidence for a specific …
F Romagné, MA Peyrat, C Leget, F Davodeau… - Journal of …, 1996 - Elsevier
We recently showed that secretion of non-chimeric disulfide-linked human γδ TCR
('soluble'TCR, sTCR) comprising Vγ9 and Vδ2 regions could be achieved by simply
introducing translational termination codons upstream from the sequences encoding TCR
transmembrane region. Here we extended these findings by demonstrating efficient
secretion and heterodimerization of γδ sTCR comprising Vγ8, Vδ1 and Vδ3 regions,
obtained via the same strategy. After immunization against immunoaffinity-purified soluble …
('soluble'TCR, sTCR) comprising Vγ9 and Vδ2 regions could be achieved by simply
introducing translational termination codons upstream from the sequences encoding TCR
transmembrane region. Here we extended these findings by demonstrating efficient
secretion and heterodimerization of γδ sTCR comprising Vγ8, Vδ1 and Vδ3 regions,
obtained via the same strategy. After immunization against immunoaffinity-purified soluble …