[HTML][HTML] Activation of NF-κB is involved in the survival of osteoclasts promoted by interleukin-1
E Jimi, I Nakamura, T Ikebe, S Akiyama… - Journal of Biological …, 1998 - ASBMB
We previously reported that interleukin-1 (IL-1) promoted the survival of murine osteoclast-
like cells (OCLs) formedin vitro and activated a transcription factor, NF-κB, of OCLs. The
present study examined whether the activation of NF-κB is directly involved in the survival of
OCLs promoted by IL-1. The expression of IL-1 type I receptor mRNA in OCLs was detected
by the polymerase chain reaction amplification of reverse-transcribed mRNA. An
electrophoretic mobility shift assay showed that IL-1 transiently activated NF-κB in the nuclei …
like cells (OCLs) formedin vitro and activated a transcription factor, NF-κB, of OCLs. The
present study examined whether the activation of NF-κB is directly involved in the survival of
OCLs promoted by IL-1. The expression of IL-1 type I receptor mRNA in OCLs was detected
by the polymerase chain reaction amplification of reverse-transcribed mRNA. An
electrophoretic mobility shift assay showed that IL-1 transiently activated NF-κB in the nuclei …