[HTML][HTML] 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain
T Ogihara, T Isobe, T Ichimura, M Taoka… - Journal of Biological …, 1997 - ASBMB
Insulin binding to its receptor induces the phosphorylation of cytosolic substrates, insulin
receptor substrate (IRS)-1 and IRS-2, which associate with several Src homology-2 domain-
containing proteins. To identify unique IRS-1-binding proteins, we screened a human heart
cDNA library with 32 P-labeled recombinant IRS-1 and obtained two isoforms (ε and ζ) of the
14-3-3 protein family. 14-3-3 protein has been shown to associate with IRS-1 in L6
myotubes, HepG2 hepatoma cells, Chinese hamster ovary cells, and bovine brain tissue …
receptor substrate (IRS)-1 and IRS-2, which associate with several Src homology-2 domain-
containing proteins. To identify unique IRS-1-binding proteins, we screened a human heart
cDNA library with 32 P-labeled recombinant IRS-1 and obtained two isoforms (ε and ζ) of the
14-3-3 protein family. 14-3-3 protein has been shown to associate with IRS-1 in L6
myotubes, HepG2 hepatoma cells, Chinese hamster ovary cells, and bovine brain tissue …