[HTML][HTML] The Fyn Tyrosine Kinase Binds Irs-1 and Forms a Distinct Signaling Complex during Insulin Stimulation (∗)
Irs-proteins link the receptors for insulin/IGF-1, growth hormones, and several interleukins
and interferons to signaling proteins that contain Src homology-2 (SH2). To identify new Irs-1-
binding proteins, we screened a mouse embryo expression library with recombinant [32 P]
Irs-1, which revealed a specific association between p59 fyn and Irs-1. The SH2 domain in
p59 fyn bound to phosphorylated Tyr 895 and Tyr 1172, which are located in YXX (L/I)
motifs. Mutation of p59 fyn at the COOH-terminal tyrosine phosphorylation site (Tyr 531) …
and interferons to signaling proteins that contain Src homology-2 (SH2). To identify new Irs-1-
binding proteins, we screened a mouse embryo expression library with recombinant [32 P]
Irs-1, which revealed a specific association between p59 fyn and Irs-1. The SH2 domain in
p59 fyn bound to phosphorylated Tyr 895 and Tyr 1172, which are located in YXX (L/I)
motifs. Mutation of p59 fyn at the COOH-terminal tyrosine phosphorylation site (Tyr 531) …