[HTML][HTML] p85α gene generates three isoforms of regulatory subunit for phosphatidylinositol 3-kinase (PI 3-kinase), p50α, p55α, and p85α, with different PI 3-kinase …
K Inukai, M Funaki, T Ogihara, H Katagiri… - Journal of Biological …, 1997 - ASBMB
Phosphatidylinositol 3-kinase (PI 3-kinase) is stimulated by association with a variety of
tyrosine kinase receptors and intracellular tyrosine-phosphorylated substrates. We isolated
a cDNA that encodes a 50-kDa regulatory subunit of PI 3-kinase with an expression cloning
method using 32 P-labeled insulin receptor substrate-1 (IRS-1). This 50-kDa protein
contains two SH2 domains and an inter-SH2 domain of p85α, but the SH3 and bcr homology
domains of p85α were replaced by a unique 6-amino acid sequence. Thus, this protein …
tyrosine kinase receptors and intracellular tyrosine-phosphorylated substrates. We isolated
a cDNA that encodes a 50-kDa regulatory subunit of PI 3-kinase with an expression cloning
method using 32 P-labeled insulin receptor substrate-1 (IRS-1). This 50-kDa protein
contains two SH2 domains and an inter-SH2 domain of p85α, but the SH3 and bcr homology
domains of p85α were replaced by a unique 6-amino acid sequence. Thus, this protein …