Two procedures were used to isolate hepatocytic Golgi fractions from rat liver. One
procedure yields a light Golgi fraction (GF1+ 2) and the other" intact" stacks of cisternae.
Triglyceride fatty acids in nascent very low density lipoproteins (VLDL) were labeled by
injection of [3H] palmitate intravenously, and radiolabeled lipoproteins were injected as
markers of potentially contaminating endosomes. GF1+ 2 fractions were enriched manyfold
in the endosomal markers, indicative of substantial endosomal contamination, whereas …