Neurotensin stimulates exocytotic histamine secretion from rat mast cells and elevates plasma histamine levels.
R Carraway, DE Cochrane, JB Lansman… - The Journal of …, 1982 - Wiley Online Library
R Carraway, DE Cochrane, JB Lansman, SE Leeman, BM Paterson, HJ Welch
The Journal of Physiology, 1982•Wiley Online Library1. Neurotensin stimulated histamine release and granule extrusion when applied to isolated
rat peritoneal mast cells. 2. This secretory response was prevented by the removal of
calcium or energy and was not accompanied by the release of lactic dehydrogenase. 3. The
secretory response produced by neurotensin was prevented by prior treatment of mast cells
with cromoglycate. 4. The intravenous injection of neurotensin into anaesthetized rats
produced a rapid and significant increase in the level of blood histamine that was dependent …
rat peritoneal mast cells. 2. This secretory response was prevented by the removal of
calcium or energy and was not accompanied by the release of lactic dehydrogenase. 3. The
secretory response produced by neurotensin was prevented by prior treatment of mast cells
with cromoglycate. 4. The intravenous injection of neurotensin into anaesthetized rats
produced a rapid and significant increase in the level of blood histamine that was dependent …
1. Neurotensin stimulated histamine release and granule extrusion when applied to isolated rat peritoneal mast cells. 2. This secretory response was prevented by the removal of calcium or energy and was not accompanied by the release of lactic dehydrogenase. 3. The secretory response produced by neurotensin was prevented by prior treatment of mast cells with cromoglycate. 4. The intravenous injection of neurotensin into anaesthetized rats produced a rapid and significant increase in the level of blood histamine that was dependent upon the dose of neurotensin. 5. Treatment of rats with compound 48/80, 24 hr before neurotensin, abolished the elevation in blood histamine caused by neurotensin. The intravenous injection of cromoglycate 1‐2 min before neurotensin greatly reduced the response to neurotensin. 6. The intradermal injection of neurotensin (0.03‐30 p‐mole) increased capillary permeability in rats pre‐treated intravenously with Evans Blue. This response was abolished by the antihistamine, diphenhydramine. Increasing the dose of neurotensin to 300 p‐mole partially overcame this inhibition by diphenhydramine. 7. Our results demonstrate that neurotensin can elicit an exocytotic secretory response from isolated rat peritoneal mast cells and elevate histamine levels in blood. It is suggested that some of neurotensin's physiological effects may be due to stimulation of mast cell secretion.
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