In situ polymerase chain reaction detection of viral DNA, single-copy genes, and gene rearrangements in cell suspensions and cytospins
P Komminoth, AA Long, R Ray… - Diagnostic Molecular …, 1992 - journals.lww.com
The study of low-copy viral or genomic DNA sequences by in situ hybridization (ISH) is often
limited by sensitivity. Using the polymerase chain reaction (PCR) for the amplification of
target DNA sequences in fixed cells [in situ PCR](ISPCR) before ISH, we have been able to
greatly improve the sensitivity of ISH. Viral DNA present in low copy number, single-copy
genes, as well as immunoglobulin gene rearrangements (VH3 family genes), were
successfully amplified in cells in suspension or on glass slides (cytospins). Single primer …
limited by sensitivity. Using the polymerase chain reaction (PCR) for the amplification of
target DNA sequences in fixed cells [in situ PCR](ISPCR) before ISH, we have been able to
greatly improve the sensitivity of ISH. Viral DNA present in low copy number, single-copy
genes, as well as immunoglobulin gene rearrangements (VH3 family genes), were
successfully amplified in cells in suspension or on glass slides (cytospins). Single primer …