Publisher Summary This chapter presents a collection of procedures to produce and
analyze complete mutant libraries, containing every possible single base substitution
mutation within a defined region of a DNA sequence. DNA regulatory elements (protein
binding sites) discussed in the chapter include the glucocorticoid response element (GRE)
from mouse mammary tumor virus (MMTV), an enhancer-like element from the yeast
transposable element Ty1, and the τ site at the terminus of E. coli DNA replication. The …