Cyclin A is required for the onset of DNA replication in mammalian fibroblasts

F Girard, U Strausfeld, A Fernandez, NJC Lamb - Cell, 1991 - cell.com
F Girard, U Strausfeld, A Fernandez, NJC Lamb
Cell, 1991cell.com
Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in
nontransformed mammalian flbroblasts. lnhibitlon of cyclin A synthesis or activity through
microinjection of plasmids encoding antisense cyclin A cDNA or affinity-purtfled anti-cyclin A
antlbodies during Gl phase was shown to abolish the nuclear staining for cyclin A in
plasmidinjected cells, and both procedures led to inhibition of DNA synthesis. No similar
effect was observed with injection of other antisense vectors including antisense cyclin 6 …
Summary
Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian flbroblasts. lnhibitlon of cyclin A synthesis or activity through microinjection of plasmids encoding antisense cyclin A cDNA or affinity-purtfled anti-cyclin A antlbodies during Gl phase was shown to abolish the nuclear staining for cyclin A in plasmidinjected cells, and both procedures led to inhibition of DNA synthesis. No similar effect was observed with injection of other antisense vectors including antisense cyclin 6, and reinjection of purified human cyclin A protein into cyclin A antisense-injected ceils effectively relieved this inhibition of DNA synthesis. Taken together, these data suggest that cyclin A plays a major role in the control of DNA replication in mammalian cells.
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