5-Hydroxyicosatetraenoate stimulates neutrophils by a stereospecific, G protein-linked mechanism
JT O'Flaherty, AG Rossi - Journal of Biological Chemistry, 1993 - ASBMB
We examined how 5-hydroxyicosatetraenoate (5-HETE) activates human neutrophils (PMN).
5-HETE stimulates PMN to mobilize Ca2+ but has little effect on degranulation or superoxide
anion production. It nonetheless stereospecifically induced these responses in cells primed
with tumor necrosis factor-alpha and likewise induced PMN plasma membranes to bind 35S-
labeled guanosine 5'-O-(thiotriphosphate)(GTP gamma S) and phosphohydrolyze [gamma-
32P] GTP. Pertussis toxin blocked GTP gamma S binding responses. Scatchard analyses of …
5-HETE stimulates PMN to mobilize Ca2+ but has little effect on degranulation or superoxide
anion production. It nonetheless stereospecifically induced these responses in cells primed
with tumor necrosis factor-alpha and likewise induced PMN plasma membranes to bind 35S-
labeled guanosine 5'-O-(thiotriphosphate)(GTP gamma S) and phosphohydrolyze [gamma-
32P] GTP. Pertussis toxin blocked GTP gamma S binding responses. Scatchard analyses of …