Chemiluminescence detection of nitric oxide production from rat cerebral cortical endothelial cells in culture

MK Weih, S Weikert, D Freyer, U Dirnagl - Brain Research Protocols, 1998 - Elsevier
Cultured brain microvascular endothelial cells have been used to study blood–brain barrier
properties in vitro. Among several endothelial derived factors the gaseous free radical nitric
oxide (NO) has been shown to regulate both vascular tone and blood–brain barrier
permeability. Due to its short half-life and high reactivity with many other molecules, effects
of NO have preferentially been investigated using NO synthase inhibitors, NO donors or,
more recently, in NO synthase knock-out animals. In the present study we describe a rapid …