[HTML][HTML] Protein kinase C δ inhibits the proliferation of vascular smooth muscle cells by suppressing G1 cyclin expression
S Fukumoto, Y Nishizawa, M Hosoi, H Koyama… - Journal of Biological …, 1997 - ASBMB
To elucidate the physiological role of protein kinase C (PKC) δ, a ubiquitously expressed
isoform in vascular smooth muscle cells (VSMC), PKC δ was stably overexpressed in A7r5
cells, rat clonal VSMC. The [3 H] thymidine incorporation in A7r5 overexpressed with PKC δ
(DVs) was suppressed to 37.1±16.3%(mean±SD) of the level in control or A7r5 transfected
with vector alone (EVs). The reduction of [3 H] thymidine incorporation was strongly
correlated with overexpressed PKC levels. Moreover, transient transfection of a dominant …
isoform in vascular smooth muscle cells (VSMC), PKC δ was stably overexpressed in A7r5
cells, rat clonal VSMC. The [3 H] thymidine incorporation in A7r5 overexpressed with PKC δ
(DVs) was suppressed to 37.1±16.3%(mean±SD) of the level in control or A7r5 transfected
with vector alone (EVs). The reduction of [3 H] thymidine incorporation was strongly
correlated with overexpressed PKC levels. Moreover, transient transfection of a dominant …