A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins
J Rouse, P Cohen, S Trigon, M Morange… - Cell, 1994 - cell.com
Summary MAPK-activated protein kinase-2 (MAPKAP kinase-2) is activated in vitro by the
p42 and p44 isoforms of MAPK (p42/p44-). In several cell lines, however, MAPKAP kinase-2
Is activated by sodium arsenite, heat shock, or osmotic stress and not by agonists that
activate~ 42/p44~*~. We have identified a MAPK-like enzyme that acts as a MAPKAP kinase-
2 reactivating kinase (RK). RK is recognized by an antiserum raised against a Xenopus
MAPK (Ypld), which is most similar to HOG1 from S. cerevisiae. We also identified a RK …
p42 and p44 isoforms of MAPK (p42/p44-). In several cell lines, however, MAPKAP kinase-2
Is activated by sodium arsenite, heat shock, or osmotic stress and not by agonists that
activate~ 42/p44~*~. We have identified a MAPK-like enzyme that acts as a MAPKAP kinase-
2 reactivating kinase (RK). RK is recognized by an antiserum raised against a Xenopus
MAPK (Ypld), which is most similar to HOG1 from S. cerevisiae. We also identified a RK …
Summary
MAPK-activated protein kinase-2 (MAPKAP kinase-2) is activated in vitro by the p42 and p44 isoforms of MAPK (p42/p44-). In several cell lines, however, MAPKAP kinase-2 Is activated by sodium arsenite, heat shock, or osmotic stress and not by agonists that activate~ 42/p44~*~. We have identified a MAPK-like enzyme that acts as a MAPKAP kinase-2 reactivating kinase (RK). RK is recognized by an antiserum raised against a Xenopus MAPK (Ypld), which is most similar to HOG1 from S. cerevisiae. We also identified a RK kinase (RKK) on the basis of its ability to activate either RK or a GST-Mpk2 fusion protein. The RKK, RK, and MAPKAP kinase-2 constitute a new stress-activated signal transduction pathway in vertebrates that is distinct from the classical MAPK cascade.
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