Nonenzymatic oxidative cleavage of peptide bonds in apoprotein B-100.
LG Fong, S Parthasarathy, JL Witztum… - Journal of lipid …, 1987 - ASBMB
Incubation of low density lipoprotein (LDL) with endothelial cells converts it to a form that is
avidly degraded by macrophages via the acetyl LDL receptor. This modification has
previously been shown to be accompanied by extensive breakdown of the major LDL
protein (apoB-100) to smaller peptides. ApoB-100 is known to undergo partial degradation
during isolation and purification which is commonly attributed to proteolytic enzymes derived
from plasma or to contaminant bacteria. In the present studies addition of any of ten different …
avidly degraded by macrophages via the acetyl LDL receptor. This modification has
previously been shown to be accompanied by extensive breakdown of the major LDL
protein (apoB-100) to smaller peptides. ApoB-100 is known to undergo partial degradation
during isolation and purification which is commonly attributed to proteolytic enzymes derived
from plasma or to contaminant bacteria. In the present studies addition of any of ten different …