We have established a new method capable of measuring the very low concentrations of
oxidized low density lipoprotein (OxLDL). In our previous study, we obtained a novel murine
monoclonal antibody against oxidized lipoproteins (Itabe, H. et al. 1994. J. Biol. Chem. 269:
15274-15279). The epitope of this antibody resides in oxidized products of
phosphatidylcholine that can form complexes with polypeptides, including apolipoprotein B.
When the monoclonal antibody was precoated onto microtiter wells prior to carrying out a …