[HTML][HTML] Derivation of multipotent mesenchymal precursors from human embryonic stem cells
Background Human embryonic stem cells provide access to the earliest stages of human
development and may serve as a source of specialized cells for regenerative medicine.
Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic
stem cells into tissue-restricted precursors. Methods and Findings Here, we present culture
conditions for the derivation of unlimited numbers of pure mesenchymal precursors from
human embryonic stem cells and demonstrate multilineage differentiation into fat, cartilage …
development and may serve as a source of specialized cells for regenerative medicine.
Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic
stem cells into tissue-restricted precursors. Methods and Findings Here, we present culture
conditions for the derivation of unlimited numbers of pure mesenchymal precursors from
human embryonic stem cells and demonstrate multilineage differentiation into fat, cartilage …
Background
Human embryonic stem cells provide access to the earliest stages of human development and may serve as a source of specialized cells for regenerative medicine. Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic stem cells into tissue-restricted precursors.
Methods and Findings
Here, we present culture conditions for the derivation of unlimited numbers of pure mesenchymal precursors from human embryonic stem cells and demonstrate multilineage differentiation into fat, cartilage, bone, and skeletal muscle cells.
Conclusion
Our findings will help to elucidate the mechanism of mesoderm specification during embryonic stem cell differentiation and provide a platform to efficiently generate specialized human mesenchymal cell types for future clinical applications.
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