Advertisement
Research Article Free access | 10.1172/JCI119426
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Rubanyi, G. in: JCI | PubMed | Google Scholar
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Freay, A. in: JCI | PubMed | Google Scholar
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Kauser, K. in: JCI | PubMed | Google Scholar
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Sukovich, D. in: JCI | PubMed | Google Scholar
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Burton, G. in: JCI | PubMed | Google Scholar
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Lubahn, D. in: JCI | PubMed | Google Scholar
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Couse, J. in: JCI | PubMed | Google Scholar
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Curtis, S. in: JCI | PubMed | Google Scholar
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804-0099, USA. gabor_rubanyi@berlex.com
Find articles by Korach, K. in: JCI | PubMed | Google Scholar
Published May 15, 1997 - More info
The present study was designed to test the hypothesis that estrogen receptors (ER) in the blood vessel wall play a role in the modulation of the release of endothelium-derived nitric oxide (EDNO). Both basal and stimulated release of EDNO were determined in aortic rings isolated from female and male wild-type and male homozygous estrogen receptor knock-out (ERKO) mice. 125I-17beta-estradiol binding in aortic tissue showed significantly more high affinity cytosolic- nuclear-binding sites in male compared with female wildtype mice. Estrogen receptor transcripts were present in the aorta of male wild-type mice, but they were absent in male ERKO animals. Basal release of EDNO (determined by endothelium-dependent contraction caused by NG-nitro-arginine) was significantly higher in aorta of wild-type male mice compared with wild-type female mice, and significantly lower in the aorta of male ERKO compared with male wild-type mice. Acetylcholine-induced endothelium-dependent relaxation was similar in all groups studied. No difference was observed in the activity of calcium-dependent nitric oxide synthase in homogenates of lungs and brain taken from male wild-type and ERKO mice. These studies show a significant association between the number of estrogen receptors and basal release of EDNO in the aorta of mice, and suggest that decreased vascular estrogen receptor number may represent a novel risk factor for cardiovascular diseases.