Free radical generation by early glycation products: a mechanism for accelerated atherogenesis in diabetes

Biochem Biophys Res Commun. 1990 Dec 31;173(3):932-9. doi: 10.1016/s0006-291x(05)80875-7.

Abstract

Non-enzymatic glycation of reactive amino groups in model proteins increased the rate of free radical production at physiologic pH by nearly fifty-fold over non-glycated protein. Superoxide generation was confirmed by electron paramagnetic resonance measurements with the spin-trap phenyl-t-butyl-nitrone. Both Schiff base and Amadori glycation products were found to generate free radicals in a ratio of 1:1.5. Free radicals generated by glycated protein increased peroxidation of membranes of linoleic/arachidonic acid vesicles nearly 2-fold over control, suggesting that the increased glycation of proteins in diabetes may accelerate vascular wall lipid oxidative modification.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arteriosclerosis / etiology
  • Arteriosclerosis / metabolism*
  • Catalase / pharmacology
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cyclic N-Oxides
  • Cytochrome c Group / metabolism
  • Diabetes Complications
  • Diabetes Mellitus / metabolism*
  • Electron Spin Resonance Spectroscopy
  • Free Radicals
  • Glucose / metabolism*
  • Humans
  • Hyperglycemia / complications
  • Hyperglycemia / metabolism*
  • Linoleic Acid
  • Linoleic Acids / metabolism
  • Lipid Peroxidation
  • Nitroblue Tetrazolium / metabolism
  • Nitrogen Oxides
  • Schiff Bases / metabolism
  • Spin Labels
  • Superoxide Dismutase / pharmacology

Substances

  • Cyclic N-Oxides
  • Cytochrome c Group
  • Free Radicals
  • Linoleic Acids
  • Nitrogen Oxides
  • Schiff Bases
  • Spin Labels
  • Nitroblue Tetrazolium
  • phenyl-N-tert-butylnitrone
  • Linoleic Acid
  • Catalase
  • Superoxide Dismutase
  • Glucose