In vivo alterations of IFN regulatory factor-1 and PIAS1 protein levels in cystic fibrosis epithelium
J. Clin. Invest. Thomas J. Kelley, et al. 106:403 doi:10.1172/JCI9560 [Go to this article.]

Figure 8
Stat1-mediated cell signaling in 9/HTEo^– pCEP2 and pCEPRF cells. Cells were cotransfected with pGAS-Luc and pEGFPN1 and stimulated with either 10, 25, or 100 units/mL IFN-γ or with 10% serum as described. Data were normalized for transfection efficiency as measured by GFP expression, and are presented as a ratio of relative light units (RLU) to relative fluorescence units (RFU). The number of experiments (n) is shown in parentheses above each bar. Error bars represent SEM. ^AP < 0.001; ^BP < 0.0001, pCEP2 vs. pCEPRF cells under each experimental condition, by Student’s t test. ^C,DSignificant stimulation of luciferase activity in pCEP2 cells compared with untreated (NT) pCEP2 cells as measured by Duncan’s multiple range test (^CP < 0.01; ^DP < 0.0001). ^E,FSignificant stimulation of luciferase activity in pCEPRF cells compared with untreated (NT) pCEPRF cells, as measured by Duncan’s multiple range test (^EP < 0.01; ^FP < 0.0001).