In vivo alterations of IFN regulatory factor-1 and PIAS1 protein levels in cystic fibrosis epithelium
J. Clin. Invest. Thomas J. Kelley, et al. 106:403 doi:10.1172/JCI9560 [
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Figure 7Levels of p-Stat1 coprecipitated with PIAS1. To determine if Stat1 bound to PIAS1 was phosphorylated, PIAS1 was immunoprecipitated from nasal epithelial extracts from four
CFTR+/+ and three
CFTR–/– mice (
a), and from four different preparations of 9/HTEo
– pCEP2 and pCEPRF cells (
b). Precipitates were blotted and probed for immunoreactivity with both anti-PIAS1 and anti–p-Stat1 antibodies.
