In vivo alterations of IFN regulatory factor-1 and PIAS1 protein levels in cystic fibrosis epithelium
J. Clin. Invest. Thomas J. Kelley, et al. 106:403 doi:10.1172/JCI9560 [
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Figure 6Levels of free and PIAS1-bound Stat1 in CF and non-CF mouse nasal epithelial tissue. (
a) Upper panel: Representative blot of Stat1 protein expression in excised nasal epithelium from
CFTR+/– mice (lanes 1–4) and
CFTR–/– mice (lanes 5–7). Lower panel: Free p-Stat1 levels in the same samples as determined by immunoprecipitation with anti-phosphotyrosine and probing with anti-Stat1. Each lane represents tissue isolated from individual mice. (
b) Upper panel: Levels of PIAS1 in excised nasal epithelium from
CFTR+/– mice (lanes 1–4) and
CFTR–/– mice (lanes 5–8) as determined by immunoprecipitation with anti-PIAS1 and probing with the same antibody. Lower panel: The same immunoprecipitates shown in the upper panel, probed for Stat1 levels. IP, immunoprecipitate; IB, immunoblot.
