In vivo alterations of IFN regulatory factor-1 and PIAS1 protein levels in cystic fibrosis epithelium
J. Clin. Invest. Thomas J. Kelley, et al. 106:403 doi:10.1172/JCI9560 [
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Figure 5Stat1 and PIAS1 expression in CF and non-CF model systems. (
a) Representative blot of Stat1 protein expression in excised nasal epithelium from
CFTR+/+ mice (lanes 1 and 2) and
CFTR–/– mice (lanes 3 and 4). Each lane represents tissue isolated from individual mice. (
c) PIAS1 expression in excised nasal epithelial tissue from
CFTR+/– mice (lanes 1–4) and
CFTR–/– mice (lanes 5–8). This blot was reprobed for erk as a control for protein loading. (
e) PIAS1 expression in 9/HTEo
– pCEP2 control cells (lanes 1 and 2) and CF-phenotype 9/HTEo
– pCEPRF cells (lanes 3 and 4). (
b,
d,
f) Densitometric analysis of Stat1, PIAS1, and erk expression. Number of samples (
n) is shown in parentheses above each bar. Significance determined by Student’s
t test. Error bars represent SEM.
