Dominant and recessive inheritance of morbid obesity associated with melanocortin 4 receptor deficiency
J. Clin. Invest. I. Sadaf Farooqi, et al. 106:271 doi:10.1172/JCI9397 [Go to this article.]

Figure 3
Signaling properties of mutant MC4Rs, N62S, GTins, CTCTΔ, and C271Y. HEK293 cells were transiently transfected with either empty pcDNA3 vector or the same vector expressing wild-type, CTCTΔ, GTins, N62S, and C271Y-mutant MC4Rs. The response to increasing concentrations of ligand (αMSH) was assessed by cotransfection with a cAMP-responsive reporter plasmid as described previously (18) and by measuring luciferase activity in a luminometer (see Methods). Data obtained were normalized for transfection efficiency by cotransfection of an internal control plasmid, pRL-CMV, which constitutively expresses Renilla luciferase. Data is expressed as a fold induction of luciferase activity. Each point represents the mean (± SE) of at least three independent experiments performed in quadruplicate.