Erythroid progenitors differentiate and mature in response to endogenous erythropoietin
J. Clin. Invest. Takeshi Sato, et al. 106:263 doi:10.1172/JCI9361 [Go to this article.]

Figure 8
Anti-hEPO neutralizing Ab abrogated erythropoiesis induced by SCF and sIL-6R/IL-6. CB CD34⁺ cells were cultured for 14 days in the presence of SCF plus EPO (open circles), or SCF plus sIL-6R/IL-6 (filled circles), with 0, 0.5, 5.0, or 50.0 μg/mL of anti–hEPO-neutralizing Ab. The same concentrations of rabbit IgG were added to control samples. After 7 days, when half of the medium was changed, the same concentration of anti-hEPO Ab or IgG was added. The number of hemoglobin-positive cells was calculated from total cell number and hemoglobin positivity determined by dianisidine staining. Data represent the percentage of hemoglobin-positive cells in each well with added anti-hEPO mAb relative to the corresponding control samples. Similar results were obtained in three separate experiments.