Erythroid progenitors differentiate and mature in response to endogenous erythropoietin
J. Clin. Invest. Takeshi Sato, et al. 106:263 doi:10.1172/JCI9361 [Go to this article.]

Figure 5
Detection of EPO mRNA in cells stimulated by SCF and sIL-6R/IL-6. (a) Human EPO cDNA and genomic DNA were amplified by PCR (33 cycles), and the PCR products were electrophoresed on a 7.5% polyacrylamide gel. The PCR products from hEPO cDNA and h-genomic DNA were 124 bp and 258 bp, respectively. Sizes of the markers are indicated on the left. (b) CB CD34⁺ cells were cultured in the presence of SCF and IL-6, with or without sIL-6R. After 14 days, the cells were harvested and cDNAs were synthesized after mRNA extraction. EPO mRNA was detected only in cells cultured with SCF and sIL-6R/IL-6 after 40 cycles of RT-PCR, and the sequences were confirmed.