Erythroid progenitors differentiate and mature in response to endogenous erythropoietin
J. Clin. Invest. Takeshi Sato, et al. 106:263 doi:10.1172/JCI9361 [Go to this article.]

Figure 2
Effects of AS ODN on expression of EPOR. Five hundred CB CD34⁺ cells were cultured in the presence of SCF and EPO, with or without ODNs. ODNs were initially added into cultures at 10 μM, and on day 7 of culture 5 μM ODNs were added again after half the medium was changed. After 7 subsequent days of culture, the cells were harvested and cDNAs were synthesized after mRNA extraction. EPOR and β-actin mRNAs were detected using RT-PCR (33 cycles), and the specificity of the PCR products was confirmed by sequencing. Size of the markers is indicated on the left.