Erythroid progenitors differentiate and mature in response to endogenous erythropoietin
J. Clin. Invest. Takeshi Sato, et al. 106:263 doi:10.1172/JCI9361 [Go to this article.]

Figure 1
Expression of EPOR and erythroid-specific genes. CB CD34⁺ cells were cultured in the presence of SCF plus IL-6, with or without sIL-6R, or in the presence of SCF plus EPO. After 14 days, the cells were harvested, and cDNAs were synthesized after mRNA extraction. EPOR and GPA mRNAs were detected by RT-PCR (33 cycles) in cells cultured both in the presence of SCF plus sIL-6R/IL-6 and in the presence of SCF plus EPO, but not in cells cultured in the presence of SCF plus IL-6 without sIL-6R. The specificity of each PCR product was confirmed by sequencing. Size of the markers is indicated on the left.