Local pH elevation mediated by the intrabacterial urease of Helicobacter pylori cocultured with gastric cells
J. Clin. Invest. Christoph Athmann, et al. 106:339 doi:10.1172/JCI9351 [
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Figure 1Localization of
H. pylori in coculture with AGS cells. (
a) AGS cells were stained with the dye DiSC
3(5) after 1-hour coculture with
H. pylori. BCECF-free acid was used to illuminate the bacteria after addition of urea. Both dyes were monitored simultaneously using confocal microscopy. ×63. (
b) These images show a perpendicular cut through the same sample as panel
a in the X and in the Y plane as shown by the corresponding lines of
a.
H. pylori are located primarily just above the regions of contact between the AGS cells. (
c) A higher magnification view to illustrate that the peribacterial region of individual organisms shows the change in BCECF fluorescence. ×100. The bars correspond to 3 or 10 μm as defined by the confocal software.
